Thionin - The Two - Faced Reaction
Concept: Photochemical reaction, Oxidation/reduction, and Reversable chemical reaction
Preparation: Prepare 0.001 M thionin solution by adding 100 mL distilled water to 0.023g of thionin. Stir to dissolve. The thionin has a poor shelf life; use within one week.
Introduction: So far we have been making chemical reactions by mixing varous chemicals with each other. In this demo, no new chemicals are being added. What will be added though is a light source. Let's see what happens to this purple solution when light is added.
Next I will place the solution on the overhead with half of the solution exposed to the light and half covered up. What do you think will happen?
The result is what we like to call the two faced reaction. One half stays purple, the other half has turned colorless.
Explanation: Thionin is an organic compound that can exist in an oxidized form (purple) and a reduced form (colorless).
In this experiment, light induces the reduction of thionin by iron (II) ions.
2 Fe2+ + thionin (purple) 2 H+ + light ---> 2 Fe3+ + reduced thionin-H2+ (colorless)
In the presnece of light, the iron ions give 2 electrons to the thionin and it also picks up 2 hydrogen ions to convert to the reduced form of thionin. This is an example of light energy being converted into chemical energy.
When the solution is taken away from the light, the purple color of the oxidized form of thionin rapidly returns and demonstrates an equilibrium reaction. If part of the solution is shaded, the boundry between the colorless and purple form is very sharp.
Safety: Sulfuric acid is severely corrosive to eyes and skin and is toxic. Use extreme caution when handling. Ferrous sulfate is slightly toxic by ingestion. Wear goggles and chemical resistant gloves.
Waste Disposal: The two-faced solution can be rinsed down the drain with excess water.
Resource: Philip S. Chen, Entertaining and Educational Chemical Demonstrations, Chemical Elements Publishing Co., 1974, p. 57-58.
Flinn Scientific, Chem Fax, Thionin - the Two Faced Solution, Pub. No. 0815.00.