Fructose-6-phosphate 

 

Reaction 3: Phosphate Ester Synthesis

This reaction is virtually identical to reaction 1 The fructose-6-phosphate has an alcohol group on C-1 that is reacted with phosphate from ATP to make the phosphate ester on C-1.

Again this reaction is endothermic and thus requires energy from a coupled reaction with ATP. ATP is used by being hydrolyzed to ADP and phosphate giving off energy and the phosphate for reaction with the glucose for a net loss of ATP in the overall glycolysis pathway.

Hydrolysis: ATP + H2O --> ADP + P + energy

This reaction is catalyzed by phosphofructokinase.

Fructose-1, 6-diphosphate 

Color Codes
C

H

N

O

P

S

Cl

F

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Alcohol to phosphate ester
  Reaction Changes / ball & stick
      

 

   Wireframe / ball & stick   Identify as glucose / ball & stick   Wireframe / ball & stick  
    MEP / ball & stick     MEP / ball & stick
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